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Instructions,
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BANG FLOW CONTROL AND REFILL SYSTEM INSTRUCTIONS FOR THE BANG (Bioaerosol Nebulizing Generator) FLOW CONTROL AND REFILL SYSTEMThe purpose of the flow control meter and refill system is to add fluid to the BANG without interrupting the production of aerosol. This system is used when the BANG runs out of sample fluid before the end of the designated exposure time. The BANG is normally a closed recirculating or single pass system for aerosol production and does not usually require refilling during experimental procedures. The flowmeter can also be used as an additional means of regulating the flow control system of the BANG. The Diagram shows the parts of the system and the directions of fluid flow when viewed externally. The yellow box containing the flowmeter is enlarged in the following photo and described further. This flowmeter is usually used for recirculation rates of 10 to 50 cc/min of water, but other rates such as 50 to 200 cc/min water may be used. The photo shows the back of the flowmeter with a stainless steel connector (compression fitting) at the top flow port (1) and a stainless steel T connector at the bottom port (2). The T connector (2) is further attached to both a compression fitting at (3) and the addition point for more sample (4). Arrows in the photo indicate the directions of fluid flow. Fluid recirculates by entering (4) and leaving (1) with the BANG in the center of this circuit. Additional fluid is added as needed by a syringe or syringe pump at point (3). A standard luer syringe fitting can be used to add fluid. Finally compression-fitting adapters are attached to the free ends of the tubing from points (1) and (3). These adapters are connected to the BANG as detailed next. Connect the adaptors first, and then add tubing to make a leak-proof seal. Confirm that the seal is leak proof. Please note that when finished with use of the flow controlling unit it should be rinsed with deionized water or another appropriate (plastic safe) solvent dependent upon the sample solution so that it will work properly the next time it is required. Connecting the Flowmeter and Refill system to the BANG: The Diagram below is a schematic of the refill system and BANG. (More detailed diagrams of the BANG can be found in the separate BANG instructions) First existing hose barb connectors must be removed from the BANG using an adjustable wrench. These are the usual connectors that are supplied or connected to tubing or hose for fluid recirculation in the closed system. The flowmeter system and tubing are now attached together at Points A and B in the Diagram. Thus the fluid from the top of the flowmeter enters into the top of the BANG at Point A and the fluid from the BANG leaves at Point B returning to the bottom of the flowmeter. Compression fittings attached to the free ends of the tubing from the BANG (described previously) make the above described connections secure. Finally the tubing connected at Point C in the Diagram or 4 in the photo is connected to the external fluid reservoir whether it is a syringe or syringe pump. A connector has been added to this tubing in order to seal the system when the syringe or pump is being refilled. Please note that when attaching the flowmeter assembly to the BANG it is important not to overtighten the connections to the BANG. In addition, the flowmeter must be kept in a vertical position. As the BANG operates, fluid may be added as required as described earlier. Flow may be further regulated by use of the valve at the front of the flowmeter. It is also very important to start with the valves fully open. Details of the operation of the BANG can be found in the instructions for the BANG itself. CH Technologies, Inc. Shami (7/2000) Note: Figure and photo are thumbnails - click on them to enlarge
Photo: Enlargement of flowmeter (back) from refill system. |