Dear Colleagues,

It will issue quarterly or as circumstances warrant.

For those of you that are seeking a community of interests in inhalation toxicology, add AIT to your list. In addition to the SOT’s Inhalation Toxicology Speciality Section (name subject to review) as a learned society, this group will meet your needs.. A select and active group of investigators from a number of laboratories, while currently organized in the UK, they have members throughout Europe and the USA. To my amazement, I discovered they just held their twentieth meeting. Stockholm, Sweden was the location of their meeting which was was generously supported/sponsored by Astra Zeneca. The meeting included a tour of their facilities. Registration started at noon followed by a short bus trip for all attendees a half day visit to Astra’s inhalation facility; the tour was preceded by a Swedish buffet lunch. During the tour, the able staff demonstrated their inhalation systems.

The meeting, held in Langenholmen Conference Center (formerly a Swedish prison - now that’s the ultimate in recycling - hotel rooms were actually former cells, now with all amenities). The meeting consisted of three half-day scientific sessions along with posters and exhibitors. CH Technologies, Lovelace and a number of equipment vendors were there. The first full day included sequential presentations of 30 minutes each which were given by a number of commercial and pharmaceutical labs. Joachim Heyder spoke about the issue of surface area in particle toxicology. The assembled group awarded him a CH Technologies Swiss Army knife and laser pointer for his excellent talk. A number of other individuals including the Editor made presentations. A redacted version of the programme may be found at "www.inhalation.net". It was redacted to protect the identity of individuals who might be targeted by outspoken and decidedly criminal animal rights activist’s in the UK. FYI, next year’s meeting will be in Scotland, at Inveresk Research in September, 2001.

You may contact the Editor ( jaegerr@idt.net ) to obtain information about the annual AIT meeting, the Association, membership and fees (decidedly inexpensive for exhibitors as well as attendees). I will forward your request on the person in charge. In total, it was an extraordinary scientific value, one which you will find most useful.

At the AIT meeting, Doctor Juergen Pauluhn presented a review of some of his toxicology studies. Portions of his talk are contained in a soon to be published paper. It is co-authored with Professor Ulrich Mohr of the Institute of Experimental Pathology, Hanover Medical School,Hanover, Germany. Email to Dr. Pauluhn at juergen.pauluhn.jp@bayer-ag.de may produce a copy in advance of the availability of a reprint.

See text at CH Technologies (below) for details

Transport of water, ions, and larger molecules across the pulmonary epithelium is becoming an important area of research in light of the increasing interest in using the lung as a route for the delivery of drugs to the body. The large surface area of the alveolar epithelium is composed primarily of thin, morphologically differentiated Type 1 cells. These Type 1 cells are derived from a relatively small number of Type 2 stem cells that also produce surfactant. ...

Dr. Shami’s summary is found at http://www.inhalation.net/type1_transport.htm

The long term effects of Tobramycin Solution for Inhalation (TSI) are currently being observed. TSI is commonly used in patients with cystic fibrosis (CF). CF is a genetic disease involving disordered water and salt transport in the lungs. This results in chronic bacterial bronchitis with bronchiectasis. Survival time decreases mainly due to infection with P aeruginosa. Aggressive intravenous treatment with antibiotics increases survival time in CF patients with P aeruginosa. Although it is effective in all CF patients, TSI is now known to be well tolerated and especially effective in increasing lung function in adolescents. ...

Dr. Shami’s summary is found at http://www.inhalation.net/aerosolized_antibiotic.htm

Recently, the Harvard Center for risk Analysis (HCRA) sponsored a workshop on " The Precautionary Principle," in an effort to refine it or replace it. The precautionary principle (PP) advocates the old adage "better safe than sorry" and applies it to potentially hazardous technologies and chemicals of unknown or poorly understood consequence.

Reference: Harvard Center for Risk Analysis. September 1999, 7(6)

Arlene Weiss’ summary is found at http://www.inhalation.net/assessing_risk.htm

The National Institute of Health (NIH) is considering whether to refurbish or to update of their existing peer review system. It has been argued that the foundation of NIH’s success has been its peer review system. The current system consists of small committees of nongovernment scientists, known as study sections, that judge the scientific merit of about 40,000 grant applications per year.

Source: NIH Eyes Sweeping Reform of Peer Review. Science 286(5) 1074-1076

Arlene Weiss’ summary is found at http://www.inhalation.net/peer_review.htm

"Profiles in Toxicology" is a recently added section in the journal "Toxicological Sciences", . Each month, a well known personality in the field of toxicology is remembered. In October, 2000 (vol. 57, #2), the profile of Sheldon D. Murphy appeared.

On a personal note, I am reminded that the Editor is now the last surviving member of the 1970’s Harvard School of Public Health Toxicology faculty. With high personal regard, I commend your attention to this issue of Toxicological Sciences. Dave Eaton and Lucio Costa present a fine memorial note on the life, research, scholarship and humanity of my mentor and car pool companion of many years, Sheldon Murphy.

An earlier profile of Mary O. Amdur, courtesy of an e-mail from Terry Gordon, is reprinted at http://www.inhalation.net/amdur.htm.

In addition to becoming a quarterly newsletter, this issue marks the departure from CH Technologies (USA) Inc. of our former student and professional colleague, Dr. Susan Shami. She has accepted a position with InhaleTherapeutic Systems in San Carlos, CA. She will be leaving us this month to join their organization. Susan, we wish you our very best.

We also note that Doug Cooper has "retired" for a second time. from his primary day job. First, it was from IBM and now more recently, from his most recent full time position. While he may submit materials to the staff for inclusion in the newsletter, he will no longer be listed as an editor. We thank Doug for his efforts getting this newsletter started in its formative moments.

At $8750 (base price, FOB Westwood, NJ), CH Technologies announces the availability of an affordable cigarette smoke exposure system for medical and toxicologic research. Patterned after published designs for smoke inhalation exposure, this system of directed flow inhalation exposure (mice are standard, other species are options) offers flexibility that is cost and space effective.

Two cigarettes being smoked is standard. Manual loading and lighting are standard. Automation, multiple pumps and larger numbers of cigarettes are extra cost options.

The patented Jaeger-NYU (directed flow) inhalation exposure technology is used. Exposure tubes provided are open tubes (standard). Closed animal exposure restraint tubes are also available (option). Respiratory measurements using plethysmography or the Pennock-Bergers-Jaeger system can be made during smoking (option). Almost any published protocol can be reproduced.

Mainstream (standard) smoke exposures or mixed exposures (collection and mixing of main stream plus continuous side stream smoke, option) can be performed. Six animals is the standard configuration for the base unit. Larger systems (12, 24,36, 48, 60 or 72) can be assembled for specific needs.

This manually loaded (and lighted) cigarette smoking system is driven by an automatically regulated piston pump. The type of pump used is standard in US smoke exposure studies. It offers a two second puff of 35 ml volume. The volume can be adjusted. A bell shaped profile as specified in ISO 3308 is used. Cigarettes are smoked with or without covering the aeration holes (manually set by the operator, based on insertion depth). Side stream smoke is collected for exposure use or for analysis (option).

Loaded with one or two cigarettes at a time, the piston pump can cycle once per minute (a single two second puff per FTC and ISO regulations),twice per minute, 2.3 times per minute (as required in Canadian and some US state protocols),3,4,5 or 6 times per minute.

Ignition as well as butt removal are manual (standard). Wood or paper matches, commercial butane lighter or similar ignition device, "zippo-type" lighter can be used. Semi-automatic or full automatic ignition with an electrical or gas igniter are available (option).

When a manually chosen point on the burning cigarette rod is reached (butt length), the pump is shut down and reloaded as desired. Animals may be kept in their restraint tubes until another exposure is started.

The exposure unit, smoke generator and smoke pump are available in a variety configurations. Quotations will be given based on your specific needs and space considerations.

System requires 110/200 (60-50 cycle) electricity, a compressed source suitable for breathing and an approved smoke exposure space (i.e. a hood or similar ventilated cabinet). All units are sold with a one year limited warranty.

Systems ordered by Universities and non-profit research Institutions are eligible for a 10% discount if ordered by December 31, 2000.

NOTICE -Availability, discounts, price, features and specifications are subject to continual review. Take note that changes may occur without notice.

A well known military biologic research institute has asked for and has received a 3 jet version of the BANG. It is scheduled for use with infectious agents. The device, crafted in 316 stainless steel with vertical discharge, was designed by Bob Gussman. It operates at 6 or so liters per minute (depending on pressure and liquid feed). It has 3 fluid inputs, can be used with a single or with 3 air (gas) sources and has an output similar to the 3 jet Collison. Preliminary results with Pseudomonas show higher viability (and no foaming). More information on the comparison between units will be available in the future.

Prior issues of the newsletter are at:

See the end of this document for a repeat of the previous month’s job listing. We are informed that the position may still be open.

Calendar of Upcoming Events:

The calender of current and future events appears at:

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Rudolph J. Jaeger, Ph.D., DABT + 201 666 2335 VOICE

Publisher/Editor-in-Chief + 201 666 8119 FAX

jaegerr@bellatlantic.net + 800 HOT OXIDE

Arlene J. Weiss, MS, DABT Editor, Air Pollution, Toxicology and

Epidemiology

iamalw@bellatlantic.net

Technical Leader / Study Director - Respiratory Toxicology,

Toxicology, Environmental Research and Consulting

The Dow Chemical Company

The Dow Chemical Company is a global science and technology based company that develops and manufactures a portfolio of chemical, plastic and agricultural products and services for customers in 168 countries around the world. With annual sales of more than $18 billion, Dow conducts its operations through 15 global businesses employing 39,000 people. The company has 123 manufacturing sites in 32 countries and supplies more than 3,500 products. Please visit our web site at www.dow.com.

Equal opportunity employer offering a competitive compensation and benefits package including 401k, stock purchase, performance incentives, and educational assistance.

This individual will serve as the Technical Leader and a Study Director for the Respiratory Toxicology group of the Toxicology & Environmental Research and Consulting (TERC) Laboratory. As the Technical Leader, the candidate will be the scientific expert within TERC to address issues associated with the respiratory system as a target organ. TERC is a multi-disciplinary organization, and it is anticipated that the Technical leader in respiratory toxicology will establish collaborations with the other disciplines, such as immunotoxicology, neurotoxicology, repro/developmental toxicology, etc. As a Study Director, the candidate will be responsible for the management of acute and multi-exposure inhalation toxicity studies required by various governments for the purpose of registering products for marketing and also for product stewardship purposes. Responsibilities will include leading the staff in the respiratory toxicology group in the assembly and operation of exposure chambers, test atmosphere generation, and the use and calibration of electronic data collection systems.

The candidate must have a minimum of Ph.D. (or comparable degree) in toxicology, pathology or a related field with 2 years post-doctoral experience. The candidate should have a working knowledge of techniques in inhalation toxicology and an established record of competence in the area of respiratory toxicology. This job requires an individual with a high degree of initiative who can work independently yet be able to interface with scientists and technical staff across the laboratory. The job requires the ability to work with laboratory animals (rats, mice, rabbits, guinea pigs, dogs) during both in-life and post-inlife procedures. In addition to knowledge in respiratory toxicology, this job requires strong math and computer skills, well-developed communication, prioritization and organizational skills and extremely accurate record keeping. Experience with GLP is desired. The candidate will be handling multiple projects of varying lengths with fixed deadlines.

Contact Ralph Albee, Senior Resource Leader 517-636-9087 (ralbee@dow.com) or Michael Holsapple, Research Scientist 517-636-4387 (mholsapple@dow.com) for additional information.